Figure 5.

Bnip3 induces cell death when autophagy is inhibited in Bax/Bak DKO MEFs. Cells were infected with an adenovirus encoding β-gal or Bnip3 in the presence of 10 mM 3-MA. Cell death was assessed at 24 h and 48 h by measuring LDH release (a) or PI uptake (b). Cells were cotransfected with pcDNA3.1 or Bnip3, and GFP or GFP-ATG5K130R and cell death was measured after 48 h by analyzing nuclear condensation (c) or PI uptake (d). Data are mean±S.D. (n=3, ^*P<0.05 compared with Bnip3 alone)