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Representative blot for the global analysis of proteins involved in protein lysosomal autophagy (LC3) and ubiquination (Nedd-4 and Mdm-2). Proteins were extracted from the corpora of control or sickle cell mice at three life stages (Pre-P=pre-priapism, P=priapism and Late stage-P=late stage-priapism). Loading was normalized using a protein assay prior to loading, and equal loading confirmed using the house-keeping gene, GAPDH, glyceraldehyde-3-phopshate dehydrogenase.
