Fig. 5. Co-purification of GST–Bud9p with myc-Bud8p and of GST–Bud8p with myc-Bud9p. Total protein extracts were prepared from strain RH2495 carrying either of the plasmid pairs pME1937 (myc-BUD8) and pYGEX-2T (GST), pME1937 (myc-BUD8) and pME1941 (GST-BUD9), pME1939 (myc-BUD9) and pYGEX-2T (GST), or pME1939 (myc-BUD9) and pME1940 (GST-BUD8).GST and GST fusion proteins were purified as described. Equivalent amounts of each sample were subjected to SDS–PAGE, transferred to nitrocellulose and probed with a monoclonal anti-myc antibody(α-myc) or a polyclonal anti-GST antibody (α-GST).