Fig. 6. Unipolar localization of GFP–Bud8p and GFP–Bud9p is independent of RSR1/BUD1, BUD8 or BUD9. (A) Living cells of strain RH2448 (rsr1Δ/rsr1Δ) expressing GFP–Bud8p (pME1772) or GFP–Bud9p (pME1777) grown in high ammonium media to exponential phase were viewed by DIC or by fluorescence microscopy (GFP). Identical results were obtained when expressing GFP–Bud8p or GFP–Bud9p under the control of the MET25 promoter using plasmid pME1773 or pME1778, respectively. (B) Subcellular localization of GFP–Bud8p and GFP–Bud9p in strains RH2450 (bud9Δ/bud9Δ) and RH2449 (bud8Δ/bud8Δ). Scale bar applies to (A) and (B) and represents 5 µm.