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. 2000 Dec 15;19(24):6686–6696. doi: 10.1093/emboj/19.24.6686

Table III. Plasmids used in this study.

Plasmid Description Reference
pME1766 rsr1Δ::kanR cassette for full deletion of RSR1 this study
pME1767 bud8Δ::HIS3 cassette for full deletion of BUD8 this study
pME1768 bud9Δ::HIS3 cassette for full deletion of BUD9 this study
pME1769 4.0 kb fragment containing BUD8 in pRS316 this study
pME1770 5.6 kb fragment containing BUD9 in pRS316 this study
YCp(RSR1) 1.6 kb fragment containing RSR1 in YCp50 Ruggieri et al. (1992)
pME1771 BUD8prom–GFP–BUD8 fusion in pRS316 this study
pME1772 BUD8prom–GFP–BUD8 fusion in pRS426 this study
pME1773 MET25prom–GFP–BUD8 fusion in pRS426MET25 this study
pME1775 BUD8prom–myc3–BUD8 fusion in pRS426 this study
pME1776 BUD9prom–GFP–BUD9 fusion in pRS316 this study
pME1777 BUD9prom–GFP–BUD9 fusion in pRS426 this study
pME1778 MET25prom–GFP–BUD9 fusion in pRS426MET25 this study
pME1780 BUD9prom–myc3–BUD9 fusion in pRS426 this study
pME1783 3.1 kb fragment containing BUD8 in pRS426 this study
pME1784 5.6 kb fragment containing BUD9 in pRS426 this study
pME1936 BUD8prom–myc3–BUD8 fusion in pRS306 this study
pME1937 BUD8prom–myc3–BUD8 fusion in pRS425 this study
pME1938 BUD9prom–myc3–BUD9 fusion in pRS306 this study
pME1939 BUD9prom–myc3–BUD9 fusion in pRS425 this study
pME1940 GAL1prom–GST–BUD8 fusion in pYGEX-2T this study
pME1941 GAL1prom–GST–BUD9 fusion in pYGEX-2T this study
pRS316 URA3-marked centromere vector Sikorski and Hieter (1989)
pRS426 URA3-marked 2 µm vector Christianson et al. (1992)
p426MET25 pRS426 containing MET25 promoter and CYC1 terminator Mumberg et al. (1994)
pCG38 2.6 kb fragment containing PHD1 in pRS202 Gimeno and Fink (1994)
pYGEX-2T URA3-marked 2 µm GAL1prom–GST fusion vector Schlenstedt et al. (1995)