FIGURE 4.

AKAP 10 is required for potentiation of LPS-induced NO production by endogenous and exogenous PGE₂. A, shown is mRNA expression of RI-interacting AKAPs in AMs, as determined by real time RT-PCR; data represent the mean ± S.E. from three individual experiments, each performed in triplicate, and values are expressed as -fold change relative to β-actin with AKAP1 set as 1. B, AMs were treated for 48 h with AKAP 8, 10, and 11 siRNAs or control siRNA (indicated by the dashed line), and the expression of the indicated AKAP mRNA was determined by real time RT-PCR. C, AMs were treated with target or control siRNAs as in B, and expression of the AKAP protein and of the housekeeping protein GAPDH was determined by Western blot analysis. D, cells were treated with AKAP or control siRNAs as in B followed by PGE₂ for 10 min and then LPS for another 24 h. Supernatants were harvested, and nitrite was determined. The data are the mean ± S.E. values of three separate experiments, each performed in duplicate. *, p < 0.05 versus control siRNA; #, p < 0.05 versus LPS alone; &, p < 0.05 versus PGE₂.