FIGURE 5.

Role of specific AKAPs in PGE₂ modulation of LPS-induced cytokine secretion. Cells were pretreated with Ht31 or RIAD (A, C, and E) as described in Fig. 3A or treated with control or AKAP siRNAs (B, D, and F) as described in Fig. 4B followed by PGE₂ for 10 min and then LPS for another 24 h. Supernatants were harvested, and IL-10 (A and B), IL-6 (C and D), and TNF-α (E and F) were determined by ELISA. The data are the mean ± S.E. values of three separate experiments, each performed in duplicate. *, p < 0.05 versus control siRNA without LPS; #, p < 0.05 versus control siRNA plus LPS; &, p < 0.05 versus control siRNA plus LPS plus PGE₂.