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. 2011 Jan 25;286(11):8924–8932. doi: 10.1074/jbc.M110.180638

FIGURE 2.

FIGURE 2.

Genetically preventing Aβ accumulation in 3xTg-AD mice reduces mTOR activity. A and B, representative microphotographs showing CA1 pyramidal neurons of 6-month-old 3xTg-AD and APP/Tau mice (n = 6/genotype). Sections were stained with an anti-Aβ antibody. C, representative Western blots of protein extracted from the brains of 6-month-old 3xTg-AD and APP/Tau mice (n = 6/genotype) and probed with the indicated antibodies. D, quantitative analyses of the blots show that p70S6K phosphorylation at Thr-389 (indicated as the ratio of total over phosphorylated levels) was significantly reduced in the APP/Tau mice when compared with 3xTg-AD mice. E, mTOR enzymatic activity was significantly reduced in the brains of 6-month-old APP/Tau mice when compared with age-matched 3xTg-AD mice. Notably, mTOR activity was not statistically significant between NonTg and APP/Tau mice, suggesting that high Aβ levels are necessary to induce mTOR hyperactivity in the 3xTg-AD mice. (n = 6/genotype). Data are presented as means ± S.E. *, p < 0.05.