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. 2011 Jan 12;286(11):9587–9597. doi: 10.1074/jbc.M110.202911

FIGURE 3.

FIGURE 3.

3-MA-induced inflammasome activation is dependent on NLRP3. A, BMDC from WT C57Bl/6 and NLRP3−/− mice were treated with PAM3CysK4 (PAM; 1 μg/ml), poly(I:C) (p(I:C); 10 μg/ml), LPS (10 ng/ml), or CpG (10 μg/ml) with 3-MA (10 mm) for 24 h, and supernatants were analyzed for IL-1β by ELISA. WT and caspase 1−/− (B) or ASC−/− (C) iBMM were treated with LPS (100 ng/ml) and indicated concentrations of 3MA for 24 h, and supernatants were analyzed by ELISA for IL-1β secretion. D, BMDC from WT C57Bl/6, MyD88−/−, and TRIF−/− mice were treated with LPS (10 ng/ml) plus ATP (5 mm), alum (5 mm), or indicated concentrations of 3-MA for 24 h. Supernatants were analyzed by ELISA for IL-1β. Data are means ± S.D. from one experiment representative of two (B, C, and D) or three (A). *, p < 0.05; significant difference from cells treated with TLR agonist alone (one-way ANOVA, followed by Tukey's HSD test).