FIGURE 4.

3-MA-induced inflammasome activation is dependent on ROS but not NOX2. A, BMDC were treated with LPS (10 ng/ml) and 3-MA (10 mm) with different concentrations of the reactive oxygen species scavenger NAc for 24 h, and supernatants were analyzed by ELISA for IL-1β. B, Western blot analysis of caspase 1 in cell lysates from iBMM treated with LPS (100 ng/ml) and 3-MA (at concentrations indicated) ± NAc (25 mm) for 24 h (representative of two separate experiments). ELISA analysis of IL-1β in supernatants of WT and NOX2^−/− iBMM treated with LPS (100 ng/ml) plus 3MA (C) or with LPS plus ATP (5 mm) or alum (5 mm) (D) for 24 h. Data are means ± S.D. from one experiment representative of two (C and D) or three (A). *, p < 0.05, significant difference from cells treated with LPS alone (one-way ANOVA, followed by Tukey's HSD test).