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TLR agonists induce targeting of IL-1β by autophagosomes. iBMM stably transfected with GFP-LC3 (green) were treated for 6 h with PAM3CysK4 (PAM; 1 μg/ml), poly(I:C) (p(I:C); 10 μg/ml), R837 (10 μg/ml), or CpG (10 μg/ml). Cells were fixed and stained with antibody against IL-1β (red). Nuclei are stained with bisbenzimide H 33258 (blue). Cells were analyzed by confocal microscopy. Scale bar, 10 μm.
