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Y14, but not other EJC factors, interacts with the methylosome. In all panels, HEK293 cells were transiently transfected with the empty vector (−) or the expression vector for each FLAG-tagged protein as indicated above the gels. Cell lysates (input) and anti-FLAG immunoprecipitates (IP) were analyzed by immunoblotting using antibodies against PRMT5, pICln, and FLAG.
