Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 5;286(11):8722–8728. doi: 10.1074/jbc.M110.190587

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Y14 is not methylated by PRMT5 but may modulate its methylation activity in vitro. A, GST-Y14/His-Magoh (YM, non-phosphorylated; pYM, phosphorylated) and GST-SmD1 were subjected to the in vitro methylation assay using recombinant GST-PRMT1 or immunopurified PRMT5 in the presence of ³H-labeled methyl donor and then detected by autoradiography (Auto-radio.) and Coomassie (Cooma.) Brilliant Blue staining. The lower panel shows Coomassie Blue staining of purified GST-PRMT1 (lane 1) and immunopurified FLAG-PRMT5 (lane 2). Lane 3 shows immunoblotting (IB) of the FLAG-PRMT5 co-precipitate using antibodies against PRMT5, pICln, and MEP50. The asterisk in lane 1 indicates a truncated Y14 fragment. B, in vitro methylation of GST-SmD1 using FLAG-PRMT5 immunoprecipitates was performed as in A; different amounts of GST-Y14/His-Magoh were used. Upper panel, autoradiogram; lower panel, Coomassie Blue staining.