FIGURE 4.

Y14 modulates methylation activity of PRMT5 in vivo. Total cell lysates were prepared from the following cells: mock or FLAG-Y14-expressing HEK293 cells (A), HEK293 cells that were mock-transfected or transfected with luciferase (Luc), Y14-targeting siRNAs, or the FLAG-Y14 expression vector (B), and mock or FLAG-Y14-expressing HEK293 cells that were either additionally transfected or not with an siRNA targeting PRMT5 (si-PRMT5) (C). Antibodies used for immunoblotting were as indicated to the left of each gel. The SYM10 and Y12 antibodies recognize symmetrical dimethyl-arginine.