FIGURE 9.

Increases in cellular NAD⁺ and extracellular Nampt and accumulation of SIRT1 and RelB at TNF-α promoter in septic leukocytes. A, TNF-α transcription in blood leukocytes in response to LPS stimulation. Blood leukocytes were isolated from normal and septic subjects and stimulated with LPS for 1 h. RNA was isolated and analyzed for TNF-α mRNA by real-time PCR. B, ChIP analysis of TNF-α promoter-bound SIRT1 and RelB in normal and septic leukocytes. C, intracellular NAD⁺ levels in normal (n = 3) and septic blood leukocytes (n = 12). D, extracellular Nampt levels in normal (n = 3) and septic serum (n = 12). Data in C and D are shown as mean ± S.E. N, normal control; P, septic patient.