FIGURE 3.

Self-association of Swi6 is required for efficient switching. A and B, h⁹⁰ swi6Δ and h⁹⁰ strains, respectively. The indicated strains were transformed with vector alone, nmt1-GFP-swi6, and nmt1-GFP-swi6^L315E plasmids (upper panels). Transformants were streaked for a single colony on a Pombe minimal medium with adenine (PMA) plate lacking leucine (8). Colonies formed after 3–4 days at 30 °C were stained with iodine vapors. Quantitative representation of the percentage of colonies giving dark staining with iodine (lower panels).