FIGURE 8.

Self-association of Swi6 is required for its own recruitment and setting up the histone H3-Lys-9 dimethylation mark. A, a schematic representation of the K region along with the approximate binding sites of the two sets of oligos used (14, 33). B, whole cell extracts from the swi6Δ mutant strain expressing vector alone, GFP-swi6, and GFP-swi6 ^L315E mutant plasmids were subjected to ChIP assay with α-Me2-H3-Lys-9 (center panel) and α-Swi6 (right panel) antibodies using specific sets of primers indicated in A. Representative pictures of the amplified products of the chromatin immunoprecipitated samples are shown. C and D, quantitative representation of enrichment of the regions amplified by primer sets 4 and 5 in α-Me2-H3-Lys-9 and α-Swi6 immunoprecipitated samples, respectively. E–H, whole cell extracts from the swi6⁺ strain expressing vector alone, GFP-tagged swi6, and GFP-tagged swi6 ^L315E mutant plasmids were subjected to a ChIP assay with α-Swi6 (right panel, E and F) and H3-Lys-9-Me2 (G and H) antibodies using specific sets of primers indicated in A. F and H, quantitation of the data shown in E and G, respectively.