FIGURE 4.

LYRIC is the target protein of DB16-1. A, human recombinant LYRIC protein was recognized by DB16-1 and anti-Mid in Western blotting. Similarly, four newly generated anti-LYRIC mAbs, Lyric 1-7, Lyric 2-1, Lyric 3-13, and Lyric 4-7, also bound to the LYRIC protein (left). Expression of LYRIC in COLO 205 and HUVECs was recognized by all of these antibodies (right). B, a right-shifted peak in the flow cytometry indicates DB16-1, Lyric 1-7, Lyric 2-1, Lyric 3-13, and Lyric 4-7 binding to HUVECs. CD31 and 4G2 antibodies were used as a positive and negative control, respectively. C, the binding activities of these mAbs to HUVECs were quantified and are presented as the mean ± S.D. (error bars) of three independent experiments. *, p < 0.05; **, p < 0.01.