FIGURE 5.

Identification and characterization of the B-cell epitope of DB16-1. A, phage clones with high affinity to DB16-1 were selected through ELISA assay. B, binding assay of DB16-1 with phage clones PC16-10 and HB47–1. These two phages were serially diluted from 10⁹ to 10⁴ pfu and 0 pfu. The PC16-10 bound to DB16-1 specifically, but control phage HB47–1 did not. C and D, synthetic peptide corresponding to D2NS1 protein (SP16-1) bound with DB16-1 in a dose-dependent manner. NMIgG served as the negative control. E, competitive inhibition of DB16-1 binding to D2NS1 by SP16-1 was confirmed by Western blotting. Control peptide P7M had no such effect. The values were presented as the mean ± S.D.