TABLE 1.
Distribution of genotypes and plasma glucose concentrations in the offspring of crosses of hemizygous F/A2 (F/A2tg/−) mice and heterozygous Gcn2 (Gcn2+/−) mice
Panel A: six different genotypes could be identified by PCR (F/A2tg/− and F/A2tg/tg mice could not be distinguished; F/A2−/− mice denote wild-type animals). A total of 60 mice (dead and alive) were analyzed. Four newborn mice disappeared from the nests in the first two days and could not be analyzed. Panel B: blood was collected from mice at ND1.
| Genotype (A) | Expected | Day 0 |
Day 2 |
|
|---|---|---|---|---|
| Alive | Alive | Dead | ||
| F/A2−/−/Gcn2+/+ | 4 | 0 | ||
| F/A2−/−/Gcn2+/− | 8 | 10 | 10 | |
| F/A2−/−/Gcn2−/− | 4 | 6 | 5 | 1 |
| F/A2tg/?/Gcn2+/+ | 11 | 10 | 10 | |
| F/A2tg/?/Gcn2+/− | 22 | 23 | 23 | |
| F/A2tg/?/Gcn2−/− | 11 | 7 | 0 | 7 |
| F/A2?/?/Gcn2?/? | 4 | 4 | ||
| Total | 60 | 48 | 12 | |
| Genotype (B) | Plasma glucose |
|
|---|---|---|
| mm | N | |
| F/A2−/−/Gcn2+/+ | 3.3 ± 0.2 | 23 |
| F/A2−/−/Gcn2−/− | 2.8 ± 0.3 | 7 |
| F/A2tg/?/Gcn2+/+ | 3.2 ± 0.2 | 25 |
| F/A2tg/?/Gcn2−/− | 2.7 ± 0.2 | 15 |