FIGURE 5.

Per3^−/− mice have altered body composition and glucose intolerance. A, total body mass and percent lean and fat mass were quantified using dual energy x-ray absorptiometry scanning (n = 7 wild-type and n = 7 Per3^−/−). Per3^−/− animals had higher percent body fat (p = 0.02) and lower percent lean mass (p = 0.02) compared with wild-type animals (error bars = S.D). B, histological slides of gonadal fat depots from wild-type and Per3−/− mice were prepared, and the adipocyte size was measured using MetaMorph software (Molecular Devices) from 3 mice of each genotype. Error bars represent S.E. C, after fasting for 15 h, mice (n = 8 wild type and 8 Per3^−/−) were injected with 2 mg/kg of glucose. Blood glucose was measured at time = 0, 15, 30, 60, and 120 min after injection. Per3^−/− (red) mice had glucose intolerance with higher blood sugars after the bolus compared with wild-type animals (black) (p values: 0 min = 0.9053, 15 min = 0.02, 30 min = 0.0023, 60 min = 0.001, 120 min = 0.0001; error bars represent S.E.).