Stoichiometry analysis of the OAD complex in the membrane. A, 16% SDS-PAGE of the purified recombinant OAD membrane complex. B, 6.5–12% BN-PAGE of the purified OAD membrane complex, Oad-α/γ′ complex, and Oad-α dimer. Coomassie-detected bands are marked from a to d. C, immunoblot analysis of the BN-PAGE bands from OAD using antibodies against Oad-α (α-Biotin) and Oad-γ (α-His).