FIGURE 5.

Interaction of M5 variant with C4-S. A, each catK molecule A binds two hexasaccharides: one in the same asymmetric unit (green carbon atoms) and another (violet carbon atoms) from the symmetry operation −x+1/2, y+1/2, −z+2. In turn, each hexasaccharide binds between two A molecules (in the asymmetric unit (yellow) and from the symmetry operation −x+1/2, y−1/2, −z+2, pink). As a result, a broken wave of the saccharide oligomer runs through many unit cells (see also Fig. 4A). B, the electrostatic surface of the M5 variant with two C4-S hexasaccharides shown in sticks (green carbon atoms). The M5 variant surface is largely divided into the negatively charged (red) and positively charged (blue) sides. The C4-S oligosaccharide binds to the positively charged side of the surface. The C4-S path on the surface of the WT catK is also shown (sticks, pink carbon atoms).