FIGURE 11.

Alignment of Kir2 family reveals a conserved proline located between the P-loop and the M2 helix. A, multisequence alignment of human Kir family members was performed with Clustal W (1.81) with Kir sequences from IUPHAR. Leucine 156 in Kir2.6 is instead a conserved proline that initiates the M2 transmembrane helix in nearly all other Kir channels (site is shown in bold text). B, two subunits of chicken Kir2.2 (Protein Data Bank code 3JYC) are shown, with conserved proline 156 and an adjacent disulfide bond between cysteine 155 and cysteine 123 (49).