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. 2011 Jan 5;286(11):9526–9541. doi: 10.1074/jbc.M110.170597

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Kir2.6 associates with Kir2. 1 and Kir2.2 subunits. Detergent-solubilized proteins from COS-1 cells transiently transfected with the constructs indicated were immunoprecipitated with mouse anti-HA antibodies to immunoprecipitate HA-Kir2.1 (A) or HA-Kir2.2 (B). Then the immunoblot was probed with rat anti-HA to detect immunoprecipitated HA-Kir2.1 (A, lower panel) or HA-Kir2.2 (B, lower panel) and with anti-GFP to detect coimmunoprecipitated GFP-Kir2.2 or GFP-Kir2.6 (upper panels). Inputs (left lanes) represent 2.5% of the protein used in the corresponding immunoprecipitations.