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. 2011 Jan 5;286(11):9526–9541. doi: 10.1074/jbc.M110.170597

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Kir2.6 is retained in the ER, whereas Kir2. 1 and Kir2.2 are trafficked out of the ER to the Golgi, following electroporation in mouse skeletal muscle. Mouse tibialis cranialis skeletal muscle fibers were electroporated in vivo with HA-Kir2.1, HA-Kir2.2, or Myc-Kir2.6. Seven days later, tissue was fixed, permeabilized, and labeled with rat anti-HA (or rat anti-Myc, red), mouse anti-GM130 (Golgi marker), or rabbit anti-PDI (ER marker, green) and DAPI (blue). HA-Kir2.1 and HA-Kir2.2 are trafficked to the Golgi and T-tubules, whereas Myc-Kir2.6 is retained in the ER. Bar, 20 μm.