FIGURE 9.

Kir2.2 and Kir2. 1 are partially retained in the ER following coelectroporation with Kir2.6 in mouse skeletal muscle. A, mouse tibialis cranialis skeletal muscle fibers were coelectroporated in vivo with HA-Kir2.1 and GFP-Kir2.6 or with GFP-Kir2.2 and Myc-Kir2.6. Seven days later, tissue was fixed, permeabilized, and labeled with rat anti-Myc (or rat anti-HA, red), rabbit anti-GFP (green), and DAPI (blue). Both HA-Kir2.1 and GFP-Kir2.2 are partially retained in the ER colocalized with Kir2.6. Bar, 20 μm. B, localization of Kir2.1, Kir2.2, and Kir2.6 when expressed alone or coexpressed with another Kir2.x subunit in skeletal muscle. Channel subunit distribution in skeletal muscle was visually scored for presence or absence in ER and/or Golgi from widefield fluorescence images (n = 41–102 electroporated nuclei for each condition).