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Fluorescence protease protection analysis of TPC1 and TPC2
Summary data (from n experiments) quantifying the extent (at 10 min) and initial rate of fluorescence loss for each of the indicated constructs.
| Construct | Extent | Initial rate | n |
|---|---|---|---|
| % | %/min | ||
| mRFP-TPC1 | 50 ± 6 | 7 ± 1 | 5 |
| TPC1-GFP | 75 ± 5 | 88 ± 21 | 5 |
| TPC11–225GFP | 46 ± 5 | 11 ± 1 | 5 |
| TPC11–347GFP | 52 ± 6 | 21 ± 6 | 5 |
| TPC11–628GFP | 21 ± 5 | 3 ± 1 | 5 |
| mRFP-TPC2 | 88 ± 2 | 40 ± 5 | 6 |
| TPC21–339GFP | 59 ± 5 | 13 ± 2 | 5 |
| TPC2-GFP | 60 ± 2 | 15 ± 1 | 9 |
