Table I. Expression of the gsp-lacZYA operon fusions.
| Strain | Relevant genotype | Growth temperature (°C) | β-galactosidase activity (Miller units) |
|---|---|---|---|
| PAP5033 | gspD-lacZYA | 30 | 57.6 ± 0.8 |
| PAP5033 | gspD-lacZYA | 37 | 33.4 ± 3.7 |
| PAP5064 | gspA-lacZYA | 37 | 2.4 ± 1.0 |
| PAP5108 | gspA-lacZYA hns-1001 | 37 | 8.5 ± 1.9 |
| PAP5090 | gspD-lacZYA hns-F2 | 37 | 152.3 ± 36.6 |
| PAP5048 | gspD-lacZYA hns-1001 | 37 | 147.4 ± 26.2 |
Cells were grown in LB medium to mid-exponential phase. β-galactosidase activity was measured according to Miller (Miller, 1972). The values presented are means from at least three independent measurements with standard deviations.