FIGURE 8.

Changes in PKA and AKT phosphorylation in BAT of WT and β-AR KO mice treated with saline (−) or phentolamine (10 mg/kg, intraperitoneally for WT and 8 mg/kg, intraperitoneally for β-AR KO mice). Mice were sacrificed after 30 min by cervical dislocation, and BAT was isolated for protein measurements. Phosphorylated PKA Thr¹⁹⁷ (panel A), total PKA (panel B), phosphorylated AKT Ser⁴⁷³ (panel C), and total AKT (panel D) are shown. Protein levels are normalized to GAPDH (panel E). Data are expressed as the mean ± S.E.; n = 6–7 per group. *, p < 0.05 versus all groups; +, p < 0.05 versus WT; #, p < 0.05 versus WT-saline. Food was withdrawn for 2 h before the sacrifice.