Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 10;286(11):8829–8838. doi: 10.1074/jbc.M110.166520

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version full access.

Creative Commons Attribution Non-Commercial License applies to Author Choice Articles

PMC Copyright notice

FIGURE 6. — Nedd4-1-induced regulation of Ca_v channels involves the lysosome. A, effect of MDC (100 μm for 15 h) and chloroquine (50 μm for 15 h) on Ca_v1.2α1/Ca_vβ₂/Ca_vα₂δ-1 current densities recorded in control (white bars) and Nedd4-1-transfected cells (black bars). †, p < 0.05 when compared with Nedd4-1-transfected cells treated with vehicle only; *, p < 0.05 when compared with corresponding control cells. B, surface biotinylation assays were performed in control and Nedd4-1-transfected cells treated with MDC and chloroquine. Western blots (WB) and associated bar graph, in C, show that the treatment failed to prevent the down-regulation of Ca_v1.2 channels in Nedd4-1-transfected cells. A.U., arbitrary units. The number of independent experiments is indicated in parentheses. **, p < 0.01, ***p < 0.001 when compared with control cells transfected with Ca_v1.2α1/Ca_vβ₂/Ca_vα₂δ-1 channels only. Biot., biotinylated; WCL, whole-cell lysate.