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. 2011 Jan 18;286(11):9833–9839. doi: 10.1074/jbc.M110.174128

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — AIEC traces from four rFXIII samples. A, rFXIII (trace 1), rFXIIIa° (trace 2), rFXIII^deact (trace 3), and rFXIIIa° (trace 4). rFXIIIa° (trace 2) was produced by activation in 50 mm Ca²⁺, 37 °C, 1 h. rFXIII^deact (trace 3) was produced by removal of Ca²⁺ from rFXIIIa°. rFXIIIa° from trace 4 was produced by reactivating rFXIII^deact in Ca²⁺ similarly to rFXIIIa° in trace 2. B, enlargement of the area around the retention times of rFXIII and rFXIII^deact (i.e. traces 1 and 3). rFXIII elutes as a one smooth peak whereas rFXIII^deact seems to contain at least two components.