FIGURE 5.

rFXIII activation to rFXIIIa° in 11 mm CaCl₂. Two freeze-dried rFXIII samples, heat-stressed (□) and unstressed (♦), were reconstituted and incubated at 30 °C in the presence of 11 mm CaCl₂. A, the content of rFXIIIa° was followed by AIEC. Note the difference in y axis scale compared with Fig. 4. B, the activation rates expressed as percentage rFXIIIa°/h were calculated after every injection and plotted. A subpopulation of the molecules in the heat-stressed rFXIII is more prone to activate upon addition of CaCl₂.