FIGURE 2.
The addition of PrPC-GPI increased the PrPSc content of ScN2a cells. A, The amount of PrPSc in ScN2a cells treated with different concentrations of PrPC-GPI as shown for 7 days. Values shown are the mean amount of PrPSc (ng/106 cells) ± S.D. from triplicate experiments performed 10 times (n = 30). B, ScN2a cells were incubated for 7 days with different concentrations of PrPC-GPI as shown, and cell extracts prepared (± proteinase K digestion) and separated by PAGE and transferred to membranes. β-Actin in cell extracts was detected by immunoblot with clone AC-74, and PrPSc was detected by immunoblot with mAb ICSM18.