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. 2011 Jan 6;286(11):8752–8758. doi: 10.1074/jbc.M110.186833

FIGURE 3.

FIGURE 3.

The addition of PrPC-G-lyso-PI reduced the PrPSc content of ScN2a cells. A, the amount of PrPSc in ScN2a cells treated with PrPC-G-lyso-PI as shown for 7 days. Values shown are the mean amount of PrPSc (ng/106 cells) ± S.D. from triplicate experiments performed 10 times (n = 30). B, the amount of PrPSc in SMB cells treated with PrPC-G-lyso-PI as shown for 7 days. Values shown are the mean amount of PrPSc (ng/106 cells) ± S.D. from triplicate experiments performed 8 times (n = 24). C, the amount of PrPSc in ScGT1 cells treated with PrPC-G-lyso-PI as shown for 7 days. Values shown are the mean amount of PrPSc (ng/106 cells) ± S.D. from triplicate experiments performed 8 times (n = 24). D, ScGT1 cells were incubated for 7 days with different concentrations of PrPC-G-lyso-PI as shown, cell extracts prepared (± proteinase K digestion) and separated by PAGE and transferred to membranes. β-Actin in cell extracts was detected by immunoblot with clone AC-74, and PrPSc was detected by immunoblot with mAb ICSM18.