TABLE 1.
PrPC-G-lyso-PI inhibited the conversion of PrPC to PrPSc
Prion-infected neuronal cell lines were treated daily with 10 ng of PrPC-GPI, PrPC-IPG, or PrPC-G-lyso-PI as shown. After 7 days, cells were collected, and the amount of PrPSc was measured by ELISA. Values shown are the mean amount of PrPSc (ng/106 cells) ± S.D. in ScN2a cells (n = 28), ScGT1 cells (n = 22), or SMB cells (n = 18).
| Treatment | PrPSc (ng/106 cells) |
||
|---|---|---|---|
| ScN2a cells | ScGT1 cells | SMB cells | |
| None | 1.3 ± 0.4 | 9.6 ± 1.4 | 5.8 ± 1.2 |
| PrPC-GPI | 8.7 ± 2.3 | 19.4 ± 3.1 | 15.4 ± 3.3 |
| PrPC-IPG | 1.1 ± 0.4 | 8.6 ± 2.2 | 5.2 ± 1.3 |
| PrPC-G-lyso-PI | 0.6 ± 0.3 | 3.8 ± 1.6 | 1.6 ± 1.1 |