FIGURE 3.
BH3-M6 inhibits the Bcl-XL interaction with Bad in intact cells. BH3-M6 inhibits Bcl-XL-induced GFP-Bad mitochondrial localization. COS-7 cells were grown in DMEM supplemented with 10% FBS and antibiotics, transfected with the indicated constructs, treated and analyzed as described under “Experimental Procedures.” The upper panel shows cells expressing pEGFP-Bad and pcDNA3-HA-Bcl-XL. The lower panel represents the quantification of the fluorescence data (black: diffuse, gray: punctate cells). At least 85 transfected cells per sample were counted in three different fields of view.