FIGURE 4.

Knockdown of β-arr1, Dyn1, and Dyn2 in cultured DA neurons. A, top panel: phase contrast image of a DA neuron culture. Middle panel, same field as A but showing eGFP expressed endogenously by DA neurons from transgenic TH-eGFP mice. Bottom panel, same field as A and B but showing transfection with fluorescent scrambled siRNA molecules used for control (Scr). Scale bar, 100 μm. B, agarose gels showing the product of mRNA amplification by RT-PCR after RNA extraction from DA neuron cultures transfected with Scr and either β-arr1- (top) Dyn1- (middle), or Dyn2-targeting siRNA molecules (bottom). In every example, β-actin was also amplified to normalize the results of the knockdown.