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Immunoprecipitation experiment to evaluate the loop A, C, and E contributions into the NMO-IgG epitopes. A, experiment performed with NMO-1, NMO-2, and NMO-3 sera in the AQP4 mutants described previously. Immunoprecipitated proteins were revealed with anti-AQP4 commercially available antibodies. AQP4 antibodies and multiple sclerosis (MS) sera were used as positive and negative control, respectively. B, densitometric analysis of the immunoprecipitated AQP4 described in A (n = 3).
