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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Channels (Austin). 2010 May 1;4(3):192–202. doi: 10.4161/chan.4.3.11867

Figure 7. Overexpression of Rem alters the kinetics of CDI.

Figure 7

A, Representative normalized IBa traces recorded from tsA201 cells transfected with the indicated plasmids for Vtest +5 mV. B, Representative normalized ICa traces recorded from tsA201 cells transfected with the indicated plasmids for Vtest +20 mV. C, Residual fraction of currents (r600) remaining at the end of the test pulses for Vtest +5 mV in the presence of 30 mM Ba2+. Note that there are no significant differences between conditions. D, Overexpression of Rem slows the inactivation kinetics of CDI. During an 800 ms test pulse to a series of depolarizing potentials ranging from 0 mV to 40 mV, the inactivation time constant tau was measured as described in “Materials and Methods” and the fast inactivation component taufast plotted against the corresponding depolarizing potentials. The error bars represent the standard error of the mean. Analysis of the results by student’s t test revealed a significant difference between treatments denoted by single (P<0.05) or double (P<0.00005) asterisks. Statistical comparisons are between the transfection of CaV1.2+Flag-β2a+GFP-Rem+pKH3-CaM (filled circles) and the transfection of CaV1.2+Flag-β2a+GFP+pKH3-CaM (open squares).