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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cancer Res. 2010 Nov 23;70(23):9916–9926. doi: 10.1158/0008-5472.CAN-10-0440

Figure 5. Knock-down of p21 restores in vivo tumorigenicity of DKK-1 knock-down cells.

Figure 5

Control shRNA-transduced, DKK-1 shRNA 796-transduced, DKK-1 shRNA 796/control shRNA-transduced, or DKK-1 shRNA 796-transduced/p21shRNA-transduced cells (5×105 cells/50 μl) were directly injected into the tibiae of anesthetized male nude mice (10 mice/group). Tumors were allowed to grow for 3 weeks at which time the mice were sacrificed and bones radiographed and processed for histological evaluation. A) Representative Faxitron X-rays of injected tibia. B) Percent osteolytic area of total tibial area. Radiographs from tumor-injected tibiae were digitized and the percent osteolytic area of the total tibial area was quantified. Data are presented as mean±standard error within each group; *p<0.003 vs. PC-3 control shRNA cells; +p<0.002 vs. PC-3 DKK-1 shRNA 796 cells by t-test. C) Bones were subjected to DEXA to quantify bone mineral density. Data are presented as mean±standard error within each group; *p<0.01 compared to control shRNA-transduced tibiae; +p<0.001 vs. PC-3 DKK-1 shRNA 796-transduced tibiae by t-test.

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