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. Author manuscript; available in PMC: 2012 Mar 10.
Published in final edited form as: Brain Res. 2011 Jan 15;1378:54–65. doi: 10.1016/j.brainres.2011.01.028

Figure 4.

Figure 4

Alterations in Climbing Fiber endings in the Molecular Layer of Lobule I on PN40 following ethanol exposure at 4.5 g/kg during the early postnatal period. MRC40 = mother reared control group; C4, C4-6, C7-9 = Isocaloric vehicle control groups; E4, E4-6, E7-9 = Ethanol exposure groups; (see Table 1). Error bars represent SEM.

A. VGlut2 expression intensity relative to total PC tissue volume (VG2 Intensity-Ratio). There were no significant control group differences (F(3, 20)=0.05; p > 0.05) that impact the conclusions. There was no significant interaction (F(2,29) <1) and no significant main effect of treatment (F(1,29) <1) or timing (F(2,29) <1). MRC40 = 275.81 ± 35.14.

B. Co-localized expression intensity relative to total PC tissue volume (Co-localized Intensity-Ratio). There were no significant control group differences (F(3, 20)=0.46; p > 0.05) that impact the conclusions. A significant interaction (F(2, 29) =5.1; p<0.02) was observed. Custom contrasts at the p<0.05 level identified significant differences for E4 vs C4 (*) and for E4-6 vs C4 -6(*). MRC40 = 148.88 ± 13.39.

C. VGlut2 expression volume relative to total PC tissue volume (VG2 Volume-Ratio). There were no significant control group differences (F(3, 20)=0.72; p > 0.05) that impact the conclusions. There was no significant interaction (F(2, 29) = 1.1; p>0.05) observed. A significant main effect of treatment (F(1,29) = 22.9; p<0.01) and no significant main effect of timing (F(2, 29) = 2.8; p>0.05) was observed. Custom contrasts at the p<0.05 level identified significant differences only for E4 vs C4 (*), E4 vs E7-9 (†), E4-6 vs C4-6 (*), and E4-6 vs E7-9 (†). MRC40 = 0.06 ± 0.005.

D. Co-localized expression volume relative to total PC tissue volume (Co-localized Volume-Ratio). There were no significant control group differences (F(3, 20)=1.46; p > 0.05) that impact the conclusions A significant interaction (F(2, 29) = 5.1; p<0.02) was observed. Custom contrasts at the p<0.05 level identified significant differences for E4 vs C4 (*), E4 vs E7-9 (†); E4-6 vs C4-6 (*), E4-6 vs E7-9 (†); and E7-9 vs C7-9 (*). MRC40 = 0.03 ± 0.002. .