Table 1.
Alcohol Dehydrogenase and Aldehyde Dehydrogenase Activities in Cell Extracts of HeLa Cells Transduced with ADH1B and ALDH2-Expressing Retrovirusesa
| Cell Lines | ADH | ALDH | |
|---|---|---|---|
| Low Km | High Km | ||
| HeLa | N.D | 0.6 ± 0.2 | 2.7 ± 0.6 |
| Rat hepatocyte | 14.5 ± 0.2 | 10.1 ± 0.4 | 33.3 ± 1.0 |
| H4IIEC3 | 11.3 ± 3.9 | 9.3 ± 0.3 | 10.5 ± 2.0 |
| HeLa-rat ADH | 7.8 ± 1.1 | - | - |
| HeLa-β1 ADH | 0.5 ± 0.1 | - | - |
| HeLa-β2 ADH | 4.6 ± 0.6 | - | - |
| HeLa-β3 ADH | 13.2 ± 3.5 | - | - |
| HeLa-β2 ADH +ALDH2 | 7.9 ± 1.0 | 23.7 ± 3.0 | 5.2 ± 2.5 |
| HeLa-β3 ADH +ALDH2 | 21.2 ± 1.4 | 26.9 ± 3.8 | 7.3 ± 1.6 |
a
The enzyme activities were determined as described in Methods and are reported as the mean ± standard deviation of determinations made on four different plates or suspension cultures of the noted cells. Activities are expressed as μmol/min/g protein in the cell extracts. ALDH activities were not measured in the HeLa ADH cells and are assumed to be the same as in the parent Hela cells.
ND = none detected.