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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Jan;35(1):28–38. doi: 10.1111/j.1530-0277.2010.01319.x

Table 3.

Lactate/Pyruvate Ratios in the Medium of ADH-Expressing Cells during Exposure to Ethanola

Cell Lines Without Ethanol With Ethanol
Rat hepatocyte 4.2 ± 1.0 6.4 ± 1.4
H4IIEC3 5.3 ± 1.9 7.6 ± 2.1
HeLa 20.7 ± 1.4b 21.9 ± 0.7c
HeLa-rat ADH 30.4 ± 3.5 b 33.7 ± 0.5 c
HeLa-β1 ADH 22.5 ± 1.5 b 19.1 ± 0.7 c
HeLa-β2 ADH 26.7 ± 0.9 b 27.4 ± 3.1 c
HeLa-β3 ADH 25.0 ± 0.4 b 28.7 ± 2.1 c
a

The cells were incubated with or without ethanol, in sealed flasks for 24 hours (6 hours for hepatocytes). At the end of experiment, the medium was collected and assayed for lactate and pyruvate. Lactate/pyruvate ratios are expressed as the mean ± standard deviation. The number of samples analyzed was n = 3 for rat hepatocytes and n = 4 for the others. Initial ethanol concentration was 50 mM for HeLa-β3 ADH cells and 5 mM for the others.

b

P< 0.001 compared to rat hepatocyte and H4IIEC3 without ethanol.

c

P< 0.005 compared to rat hepatocyte and H4IIEC3 with ethanol.