Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Hepatology. 2010 Dec 10;53(1):96–105. doi: 10.1002/hep.24018

Figure 6. Protein expression of Reg3b and Reg3g is downregulated in the jejunum by alcohol.

Figure 6

(A and B) Protein extracts from the proximal small intestine (jejunum) of mice on either an alcohol diet or isocaloric control diet for 3 weeks were analyzed by Western blotting with Reg3b or Reg3g antibodies. Tubulin was used as a loading control. Images are representative of one Western blot, which was reproduced in three independent experiments using different animals each time. (C) Immunohistochemical detection of Reg3b and Reg3g in paraffin-embedded proximal small intestinal sections in mice following alcohol or control feeding for 3 weeks. A representative section is shown. (D) Gene expression of human Reg3g was determined by RT-QPCR and normalized to 18S gene expression in duodenal biopsies from patients with chronic alcohol abuse (n=10) and healthy controls (n=10). Values are presented relative to healthy controls and represent the mean ± SEM. (E) Reg3g and tubulin protein expression in duodenal biopsies from patients with chronic alcohol consumption (n=6) and healthy controls (n=6) were analyzed by Western blot analysis. A representative Western blot image is shown. Densitometry of Western blot images with paired control and alcoholic samples was performed. Values are presented relative to healthy controls and represent the mean ± SEM; *p<0.05.