Table 1.
Effects of starvation and translation on the unc-103(0) phenotype.
| Genotype | Treatment | % spicule protract ed (n) |
p value Fisher’s exact test |
|---|---|---|---|
| wild type | fed 18 hrs | 10 (30) | |
| unc-103(0) | fed | 30 (439) | 0.02 vs wild type fed |
| unc-103(0) | starved | 5 (169) | 0.2 vs wild type fed |
| unc-103(0) | starved 18 hrs; fed 7 hrs | 0 (20) | 0.0016 vs unc-103(0) fed |
| unc-103(0) | starved 1 hr; fed 18 hrs | 42 (106) | 0.08 vs unc-103(0) fed |
| unc-103(0) | starved 2 hrs; fed 18 hrs | 19 (89) | 0.008 vs unc-103(0) fed |
| unc-103(0) | starved 3 hrs; fed 18hrs | 10 (59) | 0.0005 vs unc-103(0) fed |
| wild type | fed 18 hrs + 250 µg/ml cycloheximide | 3 (30) | 0.0005 vs unc-103(0) fed |
| unc-103(0) | fed 18 hrs + 250 µg/ml cycloheximide | 63 (129) | 0.0001 vs unc-103(0) fed |
| unc-103(0) | starved 18 hrs + 250 µg/ml cycloheximide | 12 (81) | 0.0006 vs unc-103(0) fed |
| unc-103(0) | starved 18 hrs + 250 µg/ml cycloheximide; fed 7hrs + 250 µg/ml cycloheximide | 34 (29) | 0.68 vs unc-103(0) fed |
| unc-103(0) | starved 18 hrs+ 250 µg/ml cycloheximide; fed7 hrs | 21 (42) | 0.28 vs unc-103(0) fed |
| unc-103(0) | starved 18 hrs; fed 7 hrs + 250 µg/ml cycloheximide plates | 0 (25) | <0.0001 vs unc-103(0) fed |
| egl-2(0) | fed | 10 (49) | 1 vs wild type |
| egl-2(0) | starved | 3 (110) | 0.1 vs egl-2(0) fed |
| egl-2(0) | starved 18 hrs; fed 7 hrs | 2 (46) | 0.2 vs egl-2(0) fed |
| egl-2(0) | fed 18 hrs + 250 µg/ml cycloheximide | 11 (44) | 1 vs egl-2(0) fed |
| egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide | 6 (90) | 0.3 vs egl-2(0) fed + cycloheximide |
| egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide; fed 7 hrs + 250 µg/ml cycloheximide | 15 (39) | 0.04 vs egl-2(0) starved 18 hrs; fed 7 hrs |
| egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide; fed 7 hrs | 13 (48) | 0.1 vs egl-2(0) starved 18 hrs; fed 7 hrs |
| egl-2(0) | starved 18 hrs; fed 7 hrs + 250 µg/ml cycloheximide plates | 6 (54) | 0.6 vs egl-2(0) starved 18 hrs; fed 7 hrs |
| unc-103(0); egl-2(0) | fed | 55 (272) | <0.0001 vs unc-103(0) |
| unc-103(0); egl-2(0) | starved | 37 (65) | 0.01 vs unc-103(0); egl-2(0) fed |
| unc-103(0); egl-2(0) | starved 18 hrs; fed 7 hrs | 33 (36) | 0.02 vs unc-103(0); egl-2(0) fed |
| unc-103(0); egl-2(0) | fed 18 hrs + 250 µg/ml cycloheximide | 84 (63) | <0.0001 vs unc-103(0); egl-2(0) fed |
| unc-103(0); egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide | 78 (116) | <0.0001 vs unc-103(0); egl-2(0) starved |
| unc-103(0); egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide; fed 7hrs + 250 µg/ml cycloheximide | 74 (38) | 0.04 vs unc-103(0); egl-2(0) fed |
| unc-103(0); egl-2(0) | starved 18 hrs + 250 µg/ml cycloheximide; fed 7 hrs | 86 (44) | <0.0001 vs unc-103(0); egl-2(0) fed |
| unc-103(0); egl-2(0) | starved 18 hrs; fed 7 hrs + 250 µg/ml cycloheximide plates | 45 (29) | 0.3 vs unc-103(0); egl-2(0) fed |