Table 3.
Mutating potential CaMKII sites on EAG K+ channel EGL-2 reduces response to transient starvation.
| % spicule protracted (n)a | ||
|---|---|---|
| Genotype | Fed | Starved |
| Wild type | 94 (50) | 75 (71)c |
| egl-2(0) | 100 (38) | 91 (55) |
| egl-2(0); rgEx398[Phsp-16∷egl-2(gf)]b | 75 (52) | 42 (36)c |
| egl-2(0); rgEx390[Phsp-16∷egl-2(gf,-CaMKII sites)]b | 100 (43) | 79 (38)c,d |
a
Protraction after 5 min in 1 mM ARE. All males were heat-shocked for 30 min @ 33°C on food and allowed to recover for 1 hrs on food prior to drug testing.
b
Three independently obtained transmitting lines were scored for each construct. One representative line is shown.
c
p value < 0.05 compared to Fed of the same genotype, Fisher’s Exact Test.
d
p value = 0.0002 to rgEx398[Phsp-16∷egl-2(gf)] starved, Fisher’s Exact Test.