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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Methods. 2010 Oct 31;7(12):973–975. doi: 10.1038/nmeth.1524

Figure 1.

Figure 1

Mapping of interacting domains of CRY2 and CIB1. (a) Schematic showing full-length CRY2 and CIB1 constructs used in experiments. The numbers below the proteins indicate amino acid residue position. (b)β-galactosidase activity of CRY2 and CIB1 constructs tested for interaction in the dark or in blue light (461 nm, 1.9 mW, 4 hr). The Gal4 binding domain (Gal4BD-X) and Gal4 activation domain (Gal4AD-Y) fusions used are indicated. The control vector was pGBKT7rec containing no insert. Error bars represent standard deviation (n = 3 samples). The inset panel shows immunoblot analysis of Gal4BD fusion proteins in yeast.