Table 3. Amino acid content on the surface of GH5 β-mannanases with known 3D structure.
| Parameters | BSP165 | BA | BSP602 | TF | ME | HJ |
| PDB code | 3jug | 2whj | 1wky | 1bqc | 2c0h | 1qno |
| Total | 56 | 55 | 56 | 53 | 63 | 74 |
| Asp (%) | 23.2 | 25.5 | 14.3 | 7.5 | 4.8 | 5.4 |
| Glu (%) | 17.9 | 18.2 | 10.7 | 7.5 | 3.2 | 1.4 |
| Negatively charged (%) | 41.1 | 43.6 | 25 | 15.1 | 7.91 | 6.8 |
| Arg (%) | 7.1 | 1.8 | 12.5 | 1.9 | 7.94 | 1.4 |
| Lys (%) | 7.1 | 7.3 | 3.6 | 7.5 | 11.1 | 6.8 |
| Positively charged (%) | 14.3 | 9.1 | 16.1 | 9.41 | 19.0 | 8.1 |
| Negatively charged/positively charged (%) | 2.9 | 4.8 | 1.6 | 1.6 | 0.4 | 0.8 |
| Asn (%) | 7.1 | 5.5 | 17.9 | 11.3 | 17.5 | 13.5 |
| Gln (%) | 7.1 | 9.1 | 5.4 | 13.2 | 6.3 | 18.9 |
| His (%) | 3.6 | 3.6 | 0 | 0 | 7.9 | 1.4 |
| Thr (%) | 1.8 | 7.3 | 3.6 | 3.8 | 4.8 | 16.2 |
| Ser (%) | 5.4 | 5.5 | 8.9 | 15.1 | 15.9 | 14.9 |
| Polar, uncharged (%) | 25 | 30.9 | 35.7 | 43.4 | 52.4 | 64.9 |
| Tyr (%) | 3.6 | 1.8 | 1.8 | 5.7 | 1.6 | 1.4 |
| Gly (%) | 0 | 1.8 | 1.8 | 3.8 | 7.9 | 8.1 |
| Pro (%) | 5.4 | 5.5 | 8.9 | 7.5 | 1.6 | 2.7 |
| Trp (%) | 5.4 | 5.5 | 1.8 | 1.9 | 3.2 | 0 |
| Leu (%) | 0 | 0 | 1.8 | 0 | 0 | 0 |
| Ile (%) | 1.8 | 0 | 1.8 | 0 | 0 | 0 |
| Val (%) | 0 | 0 | 0 | 0 | 3.2 | 0 |
| Ala (%) | 3.6 | 1.8 | 5.4 | 13.2 | 3.2 | 8.1 |
| Hydrophobic (%) | 19.6 | 16.4 | 23.2 | 32.1 | 20.6 | 20.3 |
| Asn+Gln (%) | 14.3 | 14.5 | 23.2 | 24.5 | 23.8 | 32.4 |
| Thr+Ser (%) | 7.2 | 12.8 | 12.5 | 18.9 | 20.7 | 31.1 |