Figure 5. Quantification of propidium iodide uptake in HeLa cells expressing wild-type and mutant TRPV4.

Transfected HeLa cells indicated an increase in cytotoxicity in mutant-expressing cells at 48 hours that is blocked by the TRPV channel blocker ruthenium red (RR). Data are averaged from at least 3 independent experiments. *p < 0.05, indicating significant differences when compared to WT-TRPV4 (2-tailed Student t test). Error bars, mean ± SEM.